The Araucaria Project. The Distance to the Local Group Galaxy WLM from Near-Infrared Photometry of Cepheid Variables

We have obtained deep images in the near-infrared J and K filters for several fields in the Local Group galaxy WLM. We report intensity mean magnitudes for 31 Cepheids located in these fields which we previously discovered in a wide-field optical imaging survey of WLM. The data define tight period-luminosity relations in both near-infrared bands which we use to derive the total reddening of the Cepheids in WLM and the true distance modulus of the galaxy from a multiwavelength analysis of the reddened distance moduli in the VIJK bands. From this, we obtain the values E(B-V) = 0.082 $\pm$ 0.02, and $(m-M)_{0} = 24.924 \pm 0.042$ mag, with a systematic uncertainty in the distance of about $\pm$ 3%. This Cepheid distance agrees extremely well with the distance of WLM determined from the I-band TRGB method by ourselves and others. Most of the reddening of the Cepheids in WLM (0.06 mag) is produced inside the galaxy, demonstrating again the need for an accurate determination of the total reddening and/or the use of infrared photometry to derive Cepheid distances which are accurate to 3% or better, even for small irregular galaxies like WLM.Comment: latex, 6 figures. accepted to Ap

The Araucaria Project. Near-Infrared Photometry of Cepheid Variables in the Sculptor Galaxy NGC 55

We have obtained deep images in the near-infrared J and K filters of four fields in the Sculptor Group spiral galaxy NGC 55 with the ESO VLT and ISAAC camera. For 40 long-period Cepheid variables in these fields which were recently discovered by Pietrzy{\'n}ski et al., we have determined mean J and K magnitudes from observations at two epochs, and derived distance moduli from the observed PL relations in these bands. Using these values together with the previously measured distance moduli in the optical V and I bands, we have determined a total mean reddening of the NGC 55 Cepheids of E(B-V)=0.127 $\pm$ 0.019 mag, which is mostly produced inside NGC 55 itself. For the true distance modulus of the galaxy, our multiwavelength analysis yields a value of 26.434 $\pm$ 0.037 mag (random error), corresponding to a distance of 1.94 $\pm$ 0.03 Mpc. This value is tied to an adopted true LMC distance modulus of 18.50 mag. The systematic uncertainty of our derived Cepheid distance to NGC 55 (apart from the uncertainty on the adopted LMC distance) is $\pm$4%, with the main contribution likely to come from the effect of blending of some of the Cepheids with unresolved companion stars. The distance of NGC 55 derived from our multiwavelength Cepheid analysis agrees within the errors with the distance of NGC 300, strengthening the case for a physical association of these two Sculptor Group galaxies.Comment: latex. ApJ accepte

Biomechanical Assessments of the Upper Limb for Determining Fatigue, Strain and Effort from the Laboratory to the Industrial Working Place: A Systematic Review

Recent human-centered developments in the industrial field (Industry 5.0) lead companies and stakeholders to ensure the wellbeing of their workers with assessments of upper limb performance in the workplace, with the aim of reducing work-related diseases and improving awareness of the physical status of workers, by assessing motor performance, fatigue, strain and effort. Such approaches are usually developed in laboratories and only at times they are translated to on-field applications; few studies summarized common practices for the assessments. Therefore, our aim is to review the current state-of-the-art approaches used for the assessment of fatigue, strain and effort in working scenarios and to analyze in detail the differences between studies that take place in the laboratory and in the workplace, in order to give insights on future trends and directions. A systematic review of the studies aimed at evaluating the motor performance, fatigue, strain and effort of the upper limb targeting working scenarios is presented. A total of 1375 articles were found in scientific databases and 288 were analyzed. About half of the scientific articles are focused on laboratory pilot studies investigating effort and fatigue in laboratories, while the other half are set in working places. Our results showed that assessing upper limb biomechanics is quite common in the field, but it is mostly performed with instrumental assessments in laboratory studies, while questionnaires and scales are preferred in working places. Future directions may be oriented towards multi-domain approaches able to exploit the potential of combined analyses, exploitation of instrumental approaches in workplace, targeting a wider range of people and implementing more structured trials to translate pilot studies to real practice

Cytotoxic activity of the histone deacetylase 3-Selective inhibitor Pojamide on MDA-MB-231 triple-negative breast cancer cells

We examined the effects of the ferrocene-based histone deacetylase-3 inhibitor Pojamide (N1-(2-aminophenyl)-N8-ferrocenyloctanediamide) and its two derivatives N1-(2-aminophenyl)-N6-ferrocenyladipamide and N1-(2-aminophenyl)-N8-ferroceniumoctanediamide tetrafluoroborate on triple-negative MDA-MB-231 breast cancer cells. Viability/growth assays indicated that only the first two compounds at 70 μM concentration caused an approximate halving of cell number after 24 h of exposure, whereas the tetrafluoroborate derivative exerted no effect on cell survival nor proliferation. Flow cytometric and protein blot analyses were performed on cells exposed to both Pojamide and the ferrocenyladipamide derivative to evaluate cell cycle distribution, apoptosis/autophagy modulation, and mitochondrial metabolic state in order to assess the cellular basis of the cytotoxic effect. The data obtained show that the cytotoxic effect of the two deacetylase inhibitors may be ascribed to the onset of non-apoptotic cell death conceivably linked to a down-regulation of autophagic processes and an impairment of mitochondrial function with an increase in intracellular reactive oxygen species. Our work expands the list of autophagy-regulating drugs and also provides a further example of the role played by the inhibition of autophagy in breast cancer cell death. Moreover, the compounds studied may represent attractive and promising targets for subsequent molecular modeling for anti-neoplastic agents in malignant breast cancer

The Araucaria Project. The Distance to the Sculptor dwarf spheroidal galaxy from infrared photometry of RR Lyrae stars

We have obtained single-phase near-infrared magnitudes in the J and K bands for a sample of 78 RR Lyrae stars in the Sculptor dSph galaxy. Applying different theoretical and empirical calibrations of the period-luminosity-metallicity relation for RR Lyrae stars in the infrared, we find consistent results and obtain a true, reddening-corrected distance modulus of 19.67 $\pm$ 0.02 (statistical) $\pm$ 0.12 (systematic) mag for Sculptor from our data. This distance value is consistent with the value of 19.68 $\pm$ 0.08 mag which we obtain from earlier V-band data of RR Lyrae stars in Sculptor, and the V magnitude-metallicity calibration of Sandage (1993). It is also in a very good agreement with the results obtain by Rizzi (2002) based on tip of the red giant branch (TRGB, 19.64 $\pm$ 0.08 mag) and horizontal branch (HB, 19.66 $\pm$ 0.15 mag).Comment: AJ in pres

The Araucaria Project. An improved distance to the Sculptor spiral galaxy NGC 300 from its Cepheid variables

In a previous paper, we reported on the discovery of more than a hundred new Cepheid variables in the Sculptor Group spiral NGC 300 from wide-field images taken in the B and V photometric bands at ESO/La Silla. In this paper, we present additional VI data, derive improved periods and mean magnitudes for the variables, and construct period-luminosity relations in the V, I and the reddening-independent (V-I) Wesenheit bands using 58 Cepheid variables with periods between 11 and 90 days. We obtain tightly defined relations, and by fitting the slopes determined for the LMC Cepheids by the OGLE II Project we obtain reddening-corrected distances to the galaxy in all bands. We adopt as our best value the distance derived from the reddening-free Wesenheit magnitudes, which is 26.43 $\pm$ 0.04 (random) $\pm$ 0.05 (systematic) mag. We argue that our current distance result for NGC 300 is the most accurate which has so far been obtained using Cepheid variables, and that it is largely free from systematic effects due to metallicity, blending, and sample selection. It agrees very well with the recent distance determination from the tip of the red giant branch method obtained from HST data by Butler et al. (2004), and it is consistent with the Cepheid distance to NGC 300 which was derived by Freedman et al. (2001) from CCD photometry of a smaller sample of stars.Comment: Latex, Astronomical Journal in pres

Electrolyte profiles with induced hypothermia : A sub study of a clinical trial evaluating the duration of hypothermia after cardiac arrest

Background Electrolyte disturbances can result from targeted temperature treatment (TTM) in out-of-hospital cardiac arrest (OHCA) patients. This study explores electrolyte changes in blood and urine in OHCA patients treated with TTM. Methods This is a sub-study of the TTH48 trial, with the inclusion of 310 unconscious OHCA patients treated with TTM at 33 degrees C for 24 or 48 h. Over a three-day period, serum concentrations were obtained on sodium potassium, chloride, ionized calcium, magnesium and phosphate, as were results from a 24-h diuresis and urine electrolyte concentration and excretion. Changes over time were analysed with a mixed-model multivariate analysis of variance with repeated measurements. Results On admission, mean +/- SD sodium concentration was 138 +/- 3.5 mmol/l, which increased slightly but significantly (p < .05) during the first 24 h. Magnesium concentration stayed within the reference interval. Median ionized calcium concentration increased from 1.11 (IQR 1.1-1.2) mmol/l during the first 24 h (p < .05), whereas median phosphate concentration dropped to 1.02 (IQR 0.8-1.2) mmol/l (p < .05) and stayed low. During rewarming, potassium concentrations increased, and magnesium and ionizes calcium concentration decreased (p < .05). Median 24-h diuresis results on days one and two were 2198 and 2048 ml respectively, and the electrolyte excretion mostly stayed low in the reference interval. Conclusions Electrolytes mostly remained within the reference interval. A temporal change occurred in potassium, magnesium and calcium concentrations with TTM's different phases. No hypothermia effect on diuresis was detected, and urine excretion of electrolytes mostly stayed low.Peer reviewe

Factors Associated With Rebound Hyperthermia After Targeted Temperature Management in Out-of- Hospital Cardiac Arrest Patients: An Explorative Substudy of the Time- Differentiated Therapeutic Hypothermia in Out-of-Hospital Cardiac Arrest Survivors Trial

Peer reviewe